Treatment of osteonecrosis of the femoral head by core decompression and implantation of fully functional ex vivo-expanded bone marrow-derived mesenchymal stem cells: a proof-of-concept study.

Stem Cells Cloning. 2019 Mar 1;12:11-16. doi: 10.2147/SCCAA.S181883. eCollection 2019.

Background: Based on several attributes involved in bone formation, bone marrow-resident mesenchymal stem cells (MSCs) have been employed in the treatment of patients suffering from femoral head osteonecrosis. Due to the low content of MSCs in the bone marrow, ex vivo expansion procedures are utilized to increase the cell number. Customarily, before administration of the resulting expanded cell product MSCs to the patient, its cellular identity is usually evaluated according to a set of «minimal phenotypic» markers, which are not modified by ex vivo processing. However, MSC functional («reparative») markers, which are severely impaired along the ex vivo expansion routine, are usually not assessed.